All lanes : Anti-PKM2 antibody (ab137852) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : H1299 whole cell lysateLane 4 : HeLa S3 whole cell lysateLane 5 : Hep G2 whole cell lysateLane 6 : MOLT4 whole cell lysateLane 7 : Raji whole cell lysateLysates/proteins at 30 µg per lane.
Immunohistochemical analysis of paraffin-embedded PKM2 xenograft tissue labelling PKM2 with ab137852 at 1/100 dilution.
Anti-PKM2 antibody (ab137852) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Anti-PKM2 antibody (ab137852) at 1/1000 dilution + Rat brain lysate/extract at 50 µg