Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling PKN1 with ab201983 at 1/200 dilution with DAB staining.
Flow cytometric analysis of HeLa cells using ab201983 at 1/200 dilution (green) and negative control (red).
Immunofluorescence analysis of HeLa cells labeling PKN1 with ab201983 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
All lanes : Anti-PKN1 antibody [4H10B1] (ab201983) at 1/500 dilutionLane 1 : HEK293 cell lysateLane 2 : PKN1 (AA: 442-620)-hIgGFc transfected HEK293 cell lysate
Anti-PKN1 antibody [4H10B1] (ab201983) at 1/500 dilution + Human PKN1 (AA: 442-620) recombinant protein