PKN1

Gene Symbol PKN1
Entrez Gene 5585
Alt Symbol DBK, PAK-1, PAK1, PKN, PKN-ALPHA, PRK1, PRKCL1
Species Human
Gene Type protein-coding
Description protein kinase N1
Other Description protease-activated kinase 1|protein kinase C-like 1|protein kinase C-like PKN|protein kinase C-related kinase 1|protein kinase PKN-alpha|protein-kinase C-related kinase 1|serine-threonine kinase N|serine-threonine protein kinase N|serine/threonine protein kinase N|serine/threonine-protein kinase N1
Swissprots Q15143 B3KVN3 A8K7W5 Q504U4 Q9UD44 Q16512 B2R9R4 Q8IUV5
Accessions EAW84419 EAW84420 EAW84421 Q16512 AK123007 BAG53845 AK292130 BAF84819 AK313886 BAG36611 BC018575 BC040061 AAH40061 BC094766 AAH94766 BQ946818 BU628889 D26181 BAA05169 DQ892477 ABM83403 S75546 AAB33345 U33053 AAC50209 XM_011528128 XP_011526430 XM_011528129 XP_011526431 NM_002741 NP_002732 NM_213560 NP_998725
Function PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser- 159',
Subcellular Location Cytoplasm. Nucleus. Endosome. Cell membrane; Peripheral membrane protein. Cleavage furrow. Midbody. Note=Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-374 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis.
Tissue Specificity Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells. {ECO:0000269|PubMed:21754995}.
Top Pathways Salmonella infection, PI3K-Akt signaling pathway

Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody - 2611 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Jurkat cells, untreated or calf intestinal alkaline phosphotase (CIP)-treated, and 293 cells, untreated or calyculin A-treated, using Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody (upper) or PRK1/PRK2 antibody (lower).

PKN (H-4) - sc-393344 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKN (H-4): sc-393344. Western blot analysis of PKN expression in Jurkat (A) and HL-60 (B) whole cell lysates.

PKN (C-19) - sc-1842 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of PKN expression in Jurkat (A,C) and H4 (B,D) whole cell lysates. Antibodies tested include PKN (N-19): sc-1843 (A), PKN (C-19): sc-1842 (B) and PKN (H-234): sc-7161 (C,D).

PKN (A-8) - sc-7969 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKN (A-8): sc-7969. Western blot analysis of PKN expression in non-transfected (A) and PKN-transfected (B) COS whole cell lysates.

PKN (H-234) - sc-7161 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PKN (H-234): sc-7161. Immunoperoxidase staining of formalin fixed, paraffin-embedded human seminal vesicle tissue showing cytoplasmic and membrane staining of glandular cells.

PKN (N-19) - sc-1843 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of PKN expression in Jurkat (A,C) and H4 (B,D) whole cell lysates. Antibodies tested include PKN (N-19): sc-1843 (A), PKN (C-19): sc-1842 (B) and PKN (H-234): sc-7161 (C,D).

PKN (49) - sc-136037 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
PKN (49): sc-136037. Western blot analysis of PKN expression in Jurkat whole cell lysate.

Anti-PKN antibody [EPR3237] - ab108976 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3237
All lanes : Anti-PKN antibody [EPR3237] (ab108976) at 1/1000 dilutionLane 1 : Jurkat lysatesLane 2 : Human spleen lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-PKN antibody [EPR3238] - ab108973 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3238
All lanes : Anti-PKN antibody [EPR3238] (ab108973) at 1/1000 dilutionLane 1 : SH-SY-5Y cell lysatesLane 2 : U937 cell lysatesLane 3 : Jurkat cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-PKN1 antibody - ab196825 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKN1 antibody (ab196825) at 1/500 dilutionLane 1 : HuvEc cell extractLane 2 : Jurkat cell extractLane 3 : Jurkat cell extract with synthesized peptide

Anti-PKN1 antibody - ab76799 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-PKN1 antibody (ab76799) at 1 µg/ml + Human liver tissue lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-PKN1 antibody - ab57424 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57424 PKN1 antibody at 1ug/ml.

Anti-PKN1 antibody [4H10B1] - ab201983 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4H10B1
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling PKN1 with ab201983 at 1/200 dilution with DAB staining.

Anti-PAK1 / PAK2 Antibody (phospho-Ser199/Ser204) - LS-C74032 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PAK1 / PAK2 Antibody (phospho-Ser144/Ser141) - LS-C74034 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PAK1 / PAK2 Antibody (phospho-Ser199/Ser204) - LS-C74033 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PAK1 / PAK2 Antibody (phospho-Thr423) - LS-C178097 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of p-PAK1/2 (T423/402) pAb in paraffin-embedded human brain tissue.

Anti-PAK1 / PAK2 Antibody (Pro429) - LS-C178183 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of PAK1/2 (P429) pAb in paraffin-embedded human brain tissue.

Anti-PKN1 Antibody (aa918-930) - LS-C17471 from LifeSpan Bioscience

Prices $455.00
Sizes 200 µg
Host Rabbit
Clonality Polyclonal

Anti-PKN1 Antibody (phospho-Thr778) - LS-C17472 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PKN1 Antibody (N-Terminus) - LS-C189174 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-PKN1 Antibody (aa911-929) - LS-C147990 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-PKN1 Antibody (aa615-874, Biotin) - LS-C300740 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PKN1 Antibody (aa615-874, FITC) - LS-C302259 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PKN1 Antibody (aa294-339) - LS-C313885 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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