Western blot analysis of extracts from HT-29 cells, asynchronous (-) or synchronized in mitosis (thymidine block, nocodazole release, mitotic shake-off; +), using Phospho-cdc25C (Thr48) (D2H3) Rabbit mAb (upper) or cdc25C (5H9) Rabbit mAb #4688 (lower).
Confocal immunofluorescent analysis of HT-29 cells, untreated (left) or λ phosphatase-treated (right), using Phospho-cdc25C (Thr48) (D2H3) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).