Anti-Plakophilin 2 antibody
| Name | Anti-Plakophilin 2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab74671 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA IHC-P |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Synthesized peptide derived from an internal region of human Plakophilin 2 |
| Description | Rabbit Polyclonal |
| Gene | PKP2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Plakophilin 2 antibody (ab74671) at 1/500 dilutionLane 1 : extracts from HeLa cellsLane 2 : extracts from HepG2 cellsLane 3 : extracts from HeLa cells with immunizing peptide at 10 µgLysates/proteins at 30 µg per lane.
IHC image of ab74671 staining in human liver cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74671, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
The spatiotemporal development of intercalated disk in three-dimensional - The spatiotemporal development of intercalated disk in three-dimensional
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Heterogeneity of ATP-sensitive K+ channels in cardiac myocytes: enrichment at the - Heterogeneity of ATP-sensitive K+ channels in cardiac myocytes: enrichment at the
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