Anti-Plectin antibody
| Name | Anti-Plectin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab83497 |
| Prices | $381.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP ELISA IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to the C terminal of Human Plectin |
| Description | Rabbit Polyclonal |
| Gene | PLEC |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Plectin antibody (ab83497) at 1/500 dilutionLane 1 : Mouse brain tissue lysateLane 2 : Rat brain tissue lysate
ICC/IF image of ab83497 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83497, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Variable metastatic potentials correlate with differential plectin and vimentin - Variable metastatic potentials correlate with differential plectin and vimentin
Burch TC, Watson MT, Nyalwidhe JO. PLoS One. 2013 May 22;8(5):e65005.
An evaluation of oligonucleotide-based therapeutic strategies for polyQ diseases. - An evaluation of oligonucleotide-based therapeutic strategies for polyQ diseases.
Fiszer A, Olejniczak M, Switonski PM, Wroblewska JP, Wisniewska-Kruk J, Mykowska A, Krzyzosiak WJ. BMC Mol Biol. 2012 Mar 7;13:6.