Anti-Pleiotrophin antibody
| Name | Anti-Pleiotrophin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55636 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 45-155 of Human Pleiotrophin |
| Description | Mouse Monoclonal |
| Gene | PTN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Pleiotrophin antibody (ab55636) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human cerebral cortex.
ICC/IF image of ab55636 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55636, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.