Flow cytometric analysis of U251 cells labeling Pleiotrophin with ab170606 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemial analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling Pleiotrophin with ab170606 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
All lanes : Anti-Pleiotrophin antibody - C-terminal (ab170606) at 1/100 dilutionLane 1 : U251 cell lysateLane 2 : NCI-H460 cell lysateLysates/proteins at 35 µg per lane.