Anti-Plexin A1 antibody (ab32960) at 1 µg/ml + Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) at 20 µgSecondaryIR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.
(Image courtesy of Human Protein Atlas)ab32960 staining Plexin A1 protein in normal adult human kidney (as noted on Swiss Prot Q9UIW2). Brown color indicates presence of protein, blue color shows cell nuclei. Paraffin embedded human cerebral cortex tissue was incubated with ab32960 (1/20 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6..
All lanes : Anti-Plexin A1 antibody (ab32960) at 2 µg/mlLane 1 : Brain (Mouse) Tissue LysateLane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lane 4 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188)Lane 5 : Brain (Mouse) Tissue Lysate with Mouse Plexin A1 peptide (ab34127) at 2 µg/mlLane 6 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Mouse Plexin A1 peptide (ab34127) at 2 µg/mlLane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate with Mouse Plexin A1 peptide (ab34127) at 2 µg/mlLane 8 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) with Mouse Plexin A1 peptide (ab34127) at 2 µg/mlLysates/proteins at 10 µg/ml per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
Rabbit polyclonal to Plexin A1 (ab32960) used in IHC-Fr. ab32960 detecting Plexin A1 protein on PFA perfused Rat spinal cord (post injury) section. The section was incubated with 10% donkey serum in PBS for 1hour at 24°C and then stained with a 1/200 dilution of Plexin A1 (ab32960) over night at 4°C. Secondary Antibody is a donkey anti-rabbit Alexa 488 (1/1000). Plexin A1 expression visible in the motor neurons also stained with fluoro rubi.
ICC/IF image of ab32960 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab32960 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.