Anti-Plexin B2 antibody [EPR9965] (ab193355) at 1/10000 dilution + HeLa cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Plexin B2 antibody [EPR9965] (ab193355) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : U87-MG cell lysateLane 3 : 293T cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Plexin B2 antibody [EPR9965] (ab193355) at 1/1000 dilutionLane 1 : Rat brain lysateLane 2 : Raw264.7 lysateLane 3 : NIH/3T3 lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow Cytometry analysis of U87-MG cells labeling Plexin B2 using ab193355 at a 1/90 dilution (red). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (black). Unlabeled control/ colour cells without incubation with primary antibody/ secondary antibody (blue). Cells were fixed in 2% paraformaldehyde.