Anti-PLK1 (phospho T210) antibody [EPNCIR167]

• Supplier: Abcam
• Catalog: ab155095
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPNCIR167
• Applications: WB IHC-P
• Species Reactivities: Mouse, Human
• Antigen: Synthetic phospho-peptide, corresponding to residues surrounding Threonine 210 of Human PLK1 (UniProt: P53350)
• Description: Rabbit Monoclonal
• Gene: PLK1
• Conjugate: Unconjugated

Product images

ab155095 staining PLK1 (phospho T210) in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An undiluted HRP-conjugated  anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

All lanes : Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HeLa cell lysate post treatment with NocodazoleLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

All lanes : Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095) at 1/1000 dilution (unpurified)Lane 1 : HeLa cell lysate, untreatedLane 2 : HeLa cell lysate, treated with calyculin ALysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PLK1 with ab155095, unpurified, at 1/100 dilution.

Immunohistochemical analysis of paraffin-embedded Human gastroic carcinoma tissue labeling PLK1 with ab155095, unpurified, at 1/100 dilution.

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab155095, unpurified, showing +ve staining.

Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155095, unpurified, showing +ve staining.

Immunohistochemical analysis of paraffin embedded Human placenta tissue using ab155095, unpurified, showing +ve staining.