PLK1

Gene Symbol PLK1
Entrez Gene 5347
Alt Symbol PLK, STPK13
Species Human
Gene Type protein-coding
Description polo-like kinase 1
Other Description PLK-1|cell cycle regulated protein kinase|polo (Drosophia)-like kinase|polo like kinase|serine/threonine-protein kinase 13|serine/threonine-protein kinase PLK1
Swissprots P53350 Q99746 Q15153
Accessions AAB36946 CAA62260 CAS92530 CAT02195 EAW55803 EAW55804 EAW55805 EAW55806 P53350 AB084459 BAD93352 AB209179 BAD92416 AK303263 BAG64345 AK308276 AK313227 BAG36038 BC002369 AAH02369 BC003002 AAH03002 BC014846 AAH14846 DQ891045 ABM81971 DQ894224 ABM85150 L19559 AAA36659 U01038 AAA56634 X73458 CAA51837 X75932 CAA53536 NM_005030 NP_005021
Function Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL. NEDD1 phosphorylation promotes subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an i
Subcellular Location Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody. Note=During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization. Localizes onto the central spindle by phosphorylating and docking at midzone proteins KIF20A/MKLP2 and PRC1. Colocalizes with FRY to separating centrosomes and spindle poles from prophase to metaphase in mitosis, but not in other stages of the cell cycle.
Tissue Specificity Placenta and colon.
Top Pathways Cell cycle, Progesterone-mediated oocyte maturation, Oocyte meiosis

Phospho-PLK1 (Thr210) Antibody - 5472 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HT-29 cells, asynchronous or synchronized in mitosis using Phospho-PLK1 (Thr210) Antibody. The antibody was pre-incubated with a PLK1 phospho-Thr210 peptide or nonphospho-peptide as indicated. The same lysates were examined using total PLK1 (208G4) Rabbit mAb #4513.

Phospho-PLK1 (Thr210) (D5H7) Rabbit mAb - 9062 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5H7
Western blot analysis of extracts from HeLa cells, asynchronous or synchronized in mitosis, using Phospho-PLK1 (Thr210) (D5H7) Rabbit mAb (upper) or total PLK1 (208G4) Rabbit mAb #4513 (lower). Mitotic synchronization was performed by thymidine block followed by release into nocodazole and mitotic shake-off.

Phospho-PLK1 (Thr210) Antibody (ELISA-Specific) - 4354 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Validation of Phospho-PLK1 (Thr210) Antibody (ELISA-Specific) in peptide DELFIA® assay using phospho-, nonphospho-peptide controls, and DELFIA® secondary antibodies (available from Perkin Elmer Life and Analytical Sciences). At 1ug/ml the S/N=84 for Peptide 1 (VEYDGERKKT*L) , while the S/N=134 for Peptide 2 (GERKKT*LCGTPNYI), (n=2).

PLK1 Antibody - 4535 from Cell Signaling Technology

Prices $246.00
Sizes 200 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or treated with calyculin A #9902 (100 nM, 10 min), using PLK1 Antibody.

PLK1 (208G4) Rabbit mAb - 4513 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 208G4
Western blot analysis of GSK-PLK1 fusion protein and extracts from HeLa, COS and PC12 cells, using PLK1 (208G4) Rabbit mAb.

Plk (H-152) - sc-5585 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Plk (H-152): sc-5585. Western blot analysis of Plk expression in K-562 (A) and A549 (B) whole cell lysates.

Plk (N-19) - sc-6355 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Plk (N-19): sc-6355. Western blot analysis of polo-like kinase expression in K-562 (A) and Jurkat (B) whole cell lysates.

Plk (F-8) - sc-17783 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Plk (F-8): sc-17783. Western blot analysis of Plk expression in HeLa (A), A549 (B) and KNRK (C) whole cell lysates.

Plk (AZ24) - sc-53418 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Plk (AZ24): sc-53418. Western blot analysis of Plk expression in HeLa whole cell lysate.

Plk (AZ27) - sc-53419 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Plk (AZ27): sc-53419. Western blot analysis of Plk expression in non-transfected: sc-117752 (A) and human Plk transfected: sc-170528 (B) 293T whole cell lysates.

Plk (E-2) - sc-55504 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Plk (E-2): sc-55504. Western blot analysis of Plk expression in HeLa (A), JAR (B), Jurkat (C), COLO 320DM (D), A549 (E), A-431 (F) and KNRK (G) whole cell lysates.

Plk (36-298) - sc-56948 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Plk (36-298): sc-56948. Western blot analysis of Plk expression in non-transfected: sc-110760 (A) and human Plk transfected: sc-111085 (B) 293 whole cell lysates.

Plk (3F8) - sc-53751 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Plk (3F8): sc-53751. Western blot analysis of Plk expression in non-transfected: sc-110760 (A) and human Plk transfected: sc-111085 (B) 293 whole cell lysates.

p-Plk (Thr 210) - sc-135706 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
p-Plk (Thr 210): sc-135706. Western blot analysis of Plk phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) rat synaptic membrane tissue extracts.

Anti-PLK1 (phospho S137) antibody - ab21738 from Abcam

Prices $380.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab21738 stained MCF7 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21738, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PLK1 (phospho S137) antibody - ab138685 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PLK1 (phospho S137) antibody (ab138685) at 1/500 dilution + Jurkat cell extracts, treated with PMA at 30 µg

Anti-PLK1 (phospho T210) antibody - ab12157 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PLK1 (phospho T210) antibody (ab12157) at 1/500 dilution + Mouse A20 cell lysate

Anti-PLK1 (phospho T210) antibody - ab111618 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111186 at 1/50 dilution staining PLK1 in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

Anti-PLK1 (phospho T210) antibody [2A3] - ab39068 from Abcam

Prices $392.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2A3
All lanes : Anti-PLK1 (phospho T210) antibody [2A3] (ab39068) at 1 µg/mlLane 1 : Extracts from untreated Hela cellsLane 2 : Extracts from overnight nocodazole treated Hela cells

Anti-PLK1 (phospho T210) antibody [EPNCIR167] - ab155095 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPNCIR167
ab155095 staining PLK1 (phospho T210) in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An undiluted HRP-conjugated  anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

Anti-PLK1 (phospho T210) antibody [EPR2612(2)] - ab133442 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2612(2)
All lanes : Anti-PLK1 (phospho T210) antibody [EPR2612(2)] (ab133442) at 1/1000 dilutionLane 1 : Lysate of HeLa cells, untreated Lane 2 : Lysate of HeLa cells treated with nocodazoleLysates/proteins at 10 µg per lane.SecondaryHRP conjugated goat anti-rabbit antibody at 1/2000 dilution

Anti-PLK1 antibody - ab175808 from Abcam

Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling PLK1 with ab175808 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. Left - PLK1, Middle - DAPI, Right - Merge.

Anti-PLK1 antibody - ab70697 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-PLK1 antibody (ab70697) at 2 µg/mlLane 2 : Anti-PLK1 antibody (ab70697) at 1 µg/mlLane 3 : Anti-PLK1 antibody (ab70697) at 0.2 µg/mlLane 1 : Whole cell lysate from HeLa cells.Lane 2 : Whole cell lysate from HeLa cells.Lane 3 : Whole cell lysate from HeLa cells.Lysates/proteins at 50 µg per lane.

Anti-PLK1 antibody - ab61118 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of A549 cells labeling PLK1 with ab61118 at 1/100 dilution. The picture on the right is blocked with the synthesized peptide.

Anti-PLK1 antibody - ab89600 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-PLK1 antibody (ab89600) at 1 µg/ml + HeLa cell lysate at 50 µg
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