Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling PLK1 with ab175808 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. Left - PLK1, Middle - DAPI, Right - Merge.
Flow Cytometry analysis of HeLa cells labelling PLK1 with ab175808 at 1:200. Cells were fixed with 1% PFA. Cells were incuabted with the primary antibody overnight at 4°C.
Immunoprecipitation analysis of U2OS cells labelling PLK1 with ab175808. Protein A beads were crosslinked to the serum.
All lanes : Anti-PLK1 antibody (ab175808)Lane 1 : Control siLane 2 : PLK1 siLane 3 : NIH 3T3 cell lysate at 40 µgLane 4 : PC12 cell lysate at 40 µg