Anti-PLK2 antibody [EPR10070]

• Supplier: Abcam
• Catalog: ab154794
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR10070
• Applications: WB IHC-P ICC/IF ICC/IF IP FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues in Human PLK2 (UniProt Q9NYY3)
• Description: Rabbit Monoclonal
• Gene: PLK2
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab154794 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab154794, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-PLK2 antibody [EPR10070] (ab154794) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysate Lysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PLK2 with ab154794 at 1/50 dilution.

Immunofluorescent analysis of 293T cells labeling PLK2 with ab154794 at 1/100 dilution.

Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154794 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154794 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154794 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab154794 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab154794 showing +ve staining.