ICC/IF image of ab71394 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71394, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
developed using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-PLK4 antibody (ab71394) at 1 µg/mlLane 1 : Jurkat cell lysateLane 2 : NIH-3T3 cell lysateLane 3 : Mouse testis lysateLane 4 : Rat testis lysateLysates/proteins at 20 µg per lane.SecondarySwine anti-Rabbit HRP at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.