PLK4

Gene Symbol PLK4
Entrez Gene 10733
Alt Symbol MCCRP2, SAK, STK18
Species Human
Gene Type protein-coding
Description polo-like kinase 4
Other Description Snk akin kinase|serine/threonine-protein kinase 18|serine/threonine-protein kinase PLK4|serine/threonine-protein kinase Sak
Swissprots Q96Q95 Q8IYF0 B7Z837 Q9UD84 O00444 Q9UDE2 B2RAL0 B7Z8G7
Accessions EAX05196 O00444 AB006972 BAB69958 AB741646 BAP74121 AI088969 AK302858 BAH13823 AK303399 BAH13953 AK314238 BAG36907 BC036023 AAH36023 BP328161 BU633758 DC402409 DQ894189 ABM85115 Y13115 CAA73575 Z25433 CAA80920 XM_005262701 XP_005262758 XM_011531552 XP_011529854 XM_011531553 XP_011529855 NM_001190799 NP_001177728 NM_001190801 NP_001177730 NM_014264 NP_055079
Function Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activat
Subcellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Nucleus, nucleolus {ECO:0000250}. Cleavage furrow. Note=Component of the deuterosome, a structure that promotes de novo centriole amplification in multiciliated cells that can generate more than 100 centrioles. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows.

PLK4 Antibody - 3258 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Sak (H-288) - sc-98929 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Sak (H-288): sc-98929. Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic and nuclear staining of glandular cells.

Sak (T-20) - sc-49104 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Sak (P-16) - sc-49103 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Sak (N-16) - sc-49102 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Sak (19-Y7) - sc-100413 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Sak (19-Y7): sc-100413. Western blot analysis of Sak expression in HeLa nuclear extract.

Anti-PLK4 antibody - ab71394 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab71394 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71394, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PLK4 antibody - ab2642 from Abcam

Prices $378.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-PLK4 antibody (ab2642) at 2 µg/ml + human colon lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-PLK4 antibody - ab137398 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-PLK4 antibody (ab137398) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

Anti-PLK4 antibody - ab56752 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing MOLT4 cells stained with ab56752 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56752, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MOLT4 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-PLK4 / SAK Antibody (aa2-16) - LS-C55429 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (0.1 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

PLK4 Antibody - NBP1-33402 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PLK4 Antibody [NBP1-33402] - A: H1299.

PLK4 Antibody - NB100-894 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

PLK4 Antibody (1C8) - H00010733-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C8
Western Blot: PLK4 Antibody (1C8) [H00010733-M01] - Western Blot analysis of PLK4 expression in Hela NE ( Cat # L013V3 ).

PLK4 Antibody - NBP1-87095 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: PLK4 Antibody [NBP1-87095] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

PLK4 Antibody - H00010733-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

PLK4 Antibody - H00010733-D01 from Novus Biologicals

Prices $399.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

PLK4 Polyclonal Antibody - bs-6393R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SAK/STK18/PLK4 antibody produced in goat - SAB2500905 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-Sak / STK18 / PLK4: <B>Cat. No. SAB2500905</B>: (0.1 μg/mL) staining of Human Placenta lysate (35 μg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PLK4 antibody produced in rabbit - HPA035026 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-PLK4: <B>Cat. No. HPA035026</B>: Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.

Anti-PLK4 antibody produced in mouse - SAB1408790 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-PLK4: <B>Cat. No. SAB1408790</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~106.7 kDa using PLK4 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Monoclonal Anti-PLK4 antibody produced in mouse - WH0010733M1 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C8
<B>Immunoblotting</B><BR/>Monoclonal Anti-PLK4, <B>Cat. No. WH0010733M1</B> used at the antibody concentration: 1 ~ 5 μg/mL. Specific band of ~108.9 kDa using Hela NE lysate.

Sak--STK18--PLK4-Antibody - AF1953a from Abgent, a WuXi AppTec company

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
AF1953a (2 µg/ml) staining of Human Colon lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PLK4 antibody - orb100623 from Biorbyt

Prices $255.00, $340.00
Sizes 100 μg, 200 μg
Host Rabbit
Clonality Polyclonal
Immunofluorescence image of human hela cells tissue using PLK4 antibody (dilution at 2.5 ug/ml)

Goat Anti-Sak / STK18 / PLK4 Antibody (OAEB01563) - OAEB01563 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
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