ab57554 staining PLSCR3 in RAW264.7 cells from Mouse macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100n PBS + 2%BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse polyclonal(1/1000) was used as the secondary antibody.See Abreview
Western blot against tagged recombinant protein immunogen using ab57554 PLSCR3 antibody at 1ug/ml.
ab57554 at a 1/200 dilution detecting PLSCR3 in human monocytes by Flow Cytometry. The secondary used was an Alexa Fluor 488 conjugated goat anti-mouse IgG (H+L) used at a 1/500 dilution. See Abreview