Anti-Plzf antibody [5B3]
| Name | Anti-Plzf antibody [5B3] |
|---|---|
| Supplier | Abcam |
| Catalog | ab104854 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 5B3 |
| Applications | ELISA WB ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Purified recombinant fragment of Human Plzf expressed in E |
| Description | Mouse Monoclonal |
| Gene | ZBTB16 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Plzf antibody [5B3] (ab104854) at 1/500 dilution + HeLa cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/13681_Plzf-Primary-antibodies-ab104854-1.jpg)
Anti-Plzf antibody [5B3] (ab104854) at 1/500 dilution + HeLa cell lysate
Immunofluorescence analysis of Hela cells using ab104854 (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
![Overlay histogram showing K562 cells stained with ab104854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104854, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/13683_Plzf-Primary-antibodies-ab104854-3.jpg)
Overlay histogram showing K562 cells stained with ab104854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104854, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate - Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate
Lin ZY, Hirano T, Shibata S, Seki NM, Kitajima R, Sedohara A, Siomi MC, Sasaki E, Siomi H, Imamura M, Okano H. Dev Biol. 2015 Apr 1;400(1):43-58.
Arrested spermatogenesis and evidence for DNA damage in PTIP mutant testes. - Arrested spermatogenesis and evidence for DNA damage in PTIP mutant testes.
Schwab KR, Smith GD, Dressler GR. Dev Biol. 2013 Jan 1;373(1):64-71.