Anti-PMCA1 antibody
| Name | Anti-PMCA1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3528 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF ELISA ICC/IF IP WB IHC-F IHC-P |
| Species Reactivities | Mouse, Rat, Human, Pig, Primate |
| Antigen | Synthetic peptide corresponding to Rat PMCA1 aa 5-22 |
| Description | Rabbit Polyclonal |
| Gene | ATP2B1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab3528 at 1/100 staining rat brain hippocacmpal slices and slice cultures by IHC-P. The tissue was paraformaldehyde fixed, permeabilized with Triton X-100 and blocked with BSA before incubation with the antibody for 12 hours. An Alexa Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary.See Abreview
developed using the ECL techniquePerformed under reducing conditions.Observed band size : 123 kDa (why is the actual band size different from the predicted?)Exposure time : 2 minutesThis image is courtesy of an Abreview submitted by Dr R EmpsonSee Abreview
Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA1 ATPase ab3528 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA1 ATPase ab3528 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Product References
Interaction of plasma membrane Ca(2+)-ATPase isoform 4 with calcineurin A: - Interaction of plasma membrane Ca(2+)-ATPase isoform 4 with calcineurin A:
Kosiorek M, Podszywalow-Bartnicka P, Zylinska L, Zablocki K, Pikula S. Biochem Biophys Res Commun. 2011 Jul 29;411(2):235-40. doi:
Plasma membrane calcium pump (PMCA4)-neuronal nitric-oxide synthase complex - Plasma membrane calcium pump (PMCA4)-neuronal nitric-oxide synthase complex
Mohamed TM, Oceandy D, Zi M, Prehar S, Alatwi N, Wang Y, Shaheen MA, Abou-Leisa R, Schelcher C, Hegab Z, Baudoin F, Emerson M, Mamas M, Di Benedetto G, Zaccolo M, Lei M, Cartwright EJ, Neyses L. J Biol Chem. 2011 Dec 2;286(48):41520-9.
Molecular interactions of the plasma membrane calcium ATPase 2 at pre- and - Molecular interactions of the plasma membrane calcium ATPase 2 at pre- and
Garside ML, Turner PR, Austen B, Strehler EE, Beesley PW, Empson RM. Neuroscience. 2009 Aug 18;162(2):383-95.
Presynaptic plasma membrane Ca2+ ATPase isoform 2a regulates excitatory synaptic - Presynaptic plasma membrane Ca2+ ATPase isoform 2a regulates excitatory synaptic
Jensen TP, Filoteo AG, Knopfel T, Empson RM. J Physiol. 2007 Feb 15;579(Pt 1):85-99. Epub 2006 Dec 14.
Presynaptic plasma membrane Ca2+ ATPase isoform 2a regulates excitatory synaptic - Presynaptic plasma membrane Ca2+ ATPase isoform 2a regulates excitatory synaptic
Jensen TP, Filoteo AG, Knopfel T, Empson RM. J Physiol. 2007 Feb 15;579(Pt 1):85-99. Epub 2006 Dec 14.
The cell adhesion molecule neuroplastin-65 inhibits hippocampal long-term - The cell adhesion molecule neuroplastin-65 inhibits hippocampal long-term
Empson RM, Buckby LE, Kraus M, Bates KJ, Crompton MR, Gundelfinger ED, Beesley PW. J Neurochem. 2006 Nov;99(3):850-60.
Molecular determinants for differential membrane trafficking of PMCA1 and PMCA2 - Molecular determinants for differential membrane trafficking of PMCA1 and PMCA2
Grati M, Aggarwal N, Strehler EE, Wenthold RJ. J Cell Sci. 2006 Jul 15;119(Pt 14):2995-3007. Epub 2006 Jun 27.