ATP2B1

Gene Symbol ATP2B1
Entrez Gene 490
Alt Symbol PMCA1, PMCA1kb
Species Human
Gene Type protein-coding
Description ATPase, Ca++ transporting, plasma membrane 1
Other Description plasma membrane calcium pump|plasma membrane calcium-transporting ATPase 1
Swissprots Q16504 Q13819 Q12992 Q93082 Q12993 Q13820 Q13821 P20020
Accessions AAA58381 AAA58382 AAA58383 AAD09924 AAD09925 EAW97429 EAW97430 EAW97431 EAW97432 EAW97433 EAW97434 P20020 AB208896 BAD92133 AF070606 AK024895 AK027053 AK308723 AY740526 AAV41065 BC015346 AAH15346 BM974301 DQ201776 ABG24240 J04027 AAA74511 M95541 AAA35999 M95542 AAA36000 S49852 AAB24324 U15686 AAA60983 U15687 AAA60984 XM_005268919 XP_005268976 XM_011538407 XP_011536709 XM_011538408 XP_011536710 XM_011538409 XP_011536711 XM_011538410 XP_011536712 XM_011538411 XP_011536713 XM_011538412 XP_011536714 XR_944556 NM_001001323 NP_001001323 NM_001682 NP_001673
Function This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.
Subcellular Location Cell membrane; Multi-pass membrane protein.
Tissue Specificity Isoform B is ubiquitously expressed. Isoform C is found in brain cortex, skeletal muscle and heart muscle. Isoform D has only been found in fetal skeletal muscle. Isoform K has been found in small intestine and liver.
Top Pathways Calcium signaling pathway, Salivary secretion, Pancreatic secretion, Mineral absorption, Endocrine and other factor-regulated calcium reabsorption

PMCA (D-1) - sc-271193 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PMCA (D-1): sc-271193. Western blot analysis of PMCA expression in Ramos (A) and HeLa (B) whole cell lysates.

PMCA (F-3) - sc-271917 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PMCA (F-3): sc-271917. Western blot analysis of PMCA expression in HeLa (A), Ramos (B) and KNRK (C) whole cell lysates.

PMCA (H-8) - sc-271194 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PMCA (H-8): sc-271194. Western blot analysis of PMCA expression in HeLa whole cell lysate.

PMCA (H-300) - sc-28765 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PMCA (H-300): sc-28765. Immunofluorescence staining of formalin-fixed Hep G2 cells showing membrane and cytoplasmic localization.

PMCA1 (F-10) - sc-398413 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PMCA1 (F-10): sc-398413. Western blot analysis of PMCA1 expression in Hep G2 (A) and HeLa (B) whole cell lysates.

PMCA1 (C-16) - sc-16488 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PMCA1 (H-58) - sc-366883 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PMCA1 (H-58): sc-366883. Western blot analysis of PMCA1 expression in mouse brain tissue extract.

Anti-Calcium Pump pan PMCA ATPase antibody [5F10] - ab2825 from Abcam

Prices $403.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5F10
Immunocytochemistry/Immunofluorescence analysis of Calcium Pump pan PMCA ATPase shows staining in C6 cells. Calcium Pump pan PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or ab2825 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.

Anti-PMCA1 antibody - ab3528 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab3528 at 1/100 staining rat brain hippocacmpal slices and slice cultures by IHC-P. The tissue was paraformaldehyde fixed, permeabilized with Triton X-100 and blocked with BSA before incubation with the antibody for 12 hours. An Alexa Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary.See Abreview

Anti-PMCA1 antibody - ab87542 from Abcam

Host Rabbit
Clonality Polyclonal
ICC/IF image of ab87542 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87542, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PMCA1 antibody - ab54878 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab54878 Calcium Pump PMCA1 ATPase antibody at 1ug/ml.

Anti-PMCA1 antibody [EPR12029] - ab190355 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12029
All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/2000 dilutionLane 1 : U87-MG cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-ATP2B1 / PMCA1 Antibody - LS-C121394 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-ATP2B1 / PMCA1 Antibody (clone GF1) - LS-C190496 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone GF1

Anti-ATP2B1 / PMCA1 Antibody (clone 3E2) - LS-C196715 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E2
Detection limit for recombinant GST tagged ATP2B1 is approximately 0.3 ng/ml as a capture antibody.

Anti-ATP2B1 / PMCA1 Antibody (aa312-327) - LS-C154919 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ATP2B1 antibody (0.03 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PMCA1 Antibody (5F10) - NB300-578 from Novus Biologicals

Prices $299.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5F10
Western Blot: PMCA1 Antibody (5F10) [NB300-578] - Analysis of 25 ug of U251 (lane 1), human brain (lane 2) and C2C12 (lane 3).

PMCA1 Antibody - NB120-3528 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: PMCA1 Antibody [NB120-3528] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA1 ATPase or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.

PMCA1 Antibody - NBP1-46512 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

PMCA1 Antibody - NBP1-97754 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: PMCA1 Antibody [NBP1-97754] - (0.03ug/ml) Analysis of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PMCA1 Antibody (3E2.) - H00000490-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E2.
Sandwich ELISA: PMCA1 Antibody (3E2) [H00000490-M01] - Detection limit for recombinant GST tagged ATP2B1 is approximately 0.3ng/ml as a capture antibody.

Rabbit Anti-PMCA1 Polyclonal Antibody - bs-6443R from Bioss

Prices $245.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin embedded rat brain labeled with Anti-PMCA1 Polyclonal Antibody, Unconjugated (bs-6443R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Anti-ATP2B1 antibody produced in rabbit - HPA011166 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-ATP2B1: <B>Cat. No. HPA011166</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Anti-ATP2B1 antibody produced in goat - SAB2501734 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-ATP2B1: <B>Cat. No. SAB2501734</B>: (0.03 μg/mL) staining of HeLa lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ATP2B1 antibody produced in rabbit - HPA012945 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-ATP2B1: <B>Cat. No. HPA012945</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human duodenum shows strong membranous positivity in glandular cells.
Search more