Anti-Poliovirus Receptor antibody (ab103630) at 1/500 dilution + Mouse stomach tissue lysate at 50 µg
All lanes : Anti-Poliovirus Receptor antibody (ab103630) at 1/500 dilutionLane 1 : Poliovirus Receptor transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.
Immunofluorescence analysis of murine stomach, staining Poliovirus Receptor with ab103630. Cells were fixed with acetone and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in diluent) for 24 hours at 4°C. A Cy3®-conjugated sheep anti-rabbit polyclonal IgG (1/3000) was used as the secondary antibody.See Abreview