Western Blot (1μg/ml) staining of Human Liver lysate (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (3.8μg/ml) staining of paraffin embedded Human CerImmunohistochemistry ortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry (20μg/ml) staining of PFA-perfused cryosection of Porcin Kidney. Microwave antigen retrieval with citrate buffer pH 3, CY3-staining. Data obtained from Dr. Hrvoje Brzica, University of ZagrImmunohistochemistryatia