All lanes : Anti-POLR2E antibody [3C5] (ab118000) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY POLR2E cDNA Lysates/proteins at 5 µg per lane.
All lanes : Anti-POLR2E antibody [3C5] (ab118000) at 1/500 dilutionLane 1 : HepG2 lysateLane 2 : HeLa lysateLane 3 : HT29 lysateLane 4 : A549 lysateLane 5 : COS7 lysateLane 6 : Jurkat lysateLane 7 : MDCK lysateLane 8 : PC12 lysateLane 9 : MCF7 lysateLysates/proteins at 35 µg per lane.
ab118000 at 1/100 dilution staining POLR2E in COS7 cells transiently transfected by pCMV6-ENTRY POLR2E by Immunofluorescence.