All lanes : Anti-POLR2E antibody [EPR11750] (ab180151) at 1/20000 dilutionLane 1 : A431 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling POLR2E with ab180151 at 1/100 dilution, followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin.
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling POLR2E with ab180151 at 1/10 dilution followed by Goat anti rabbit IgG (FITC) at 1/75 dilution (red), compared to a Rabbit IgG control (green).
Anti-POLR2E antibody [EPR11750] (ab180151) at 1/5000 dilution + HepG2 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution