All lanes : Anti-Poly(A) RNA polymerase, mitochondrial antibody (ab156684) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 50 µgLane 4 : Jurkat whole cell lysate at 50 µgdeveloped using the ECL technique
Detection of Poly(A) RNA polymerase, mitochondrial by Western blot of Immunoprecipitate: Immunoprecipitation of HeLa whole cell lysate using ab156684 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection of Poly(A) RNA polymerase, mitochondrial with ab156684 at 1µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody. Detection: Chemiluminescence with exposure time of 30 seconds.