MTPAP

Gene Symbol	MTPAP
Entrez Gene	55149
Alt Symbol	PAPD1, SPAX4
Species	Human
Gene Type	protein-coding
Description	mitochondrial poly(A) polymerase
Other Description	PAP-associated domain-containing protein 1\|TUTase 1\|poly(A) RNA polymerase, mitochondrial\|polynucleotide adenylyltransferase\|terminal uridylyltransferase 1
Swissprots	D3DRX0 Q6P7E5 Q659E3 Q9NVV4 Q9HA74
Accessions	EAW86014 EAW86015 Q9NVV4 AB194709 BAD98252 AK001348 BAA91641 AK022188 BAB13981 AK125211 AY364242 AAQ76801 BC061703 AAH61703 DA086747 NM_018109 NP_060579
Function	Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA. {ECO:0000269\|PubMed:15547249, ECO:0000269\|PubMed:15769737, ECO:0000269\|PubMed:18172165, ECO:0000269\|PubMed:20970105, ECO:0000269\|PubMed:21292163}.
Subcellular Location	Cytoplasm. Mitochondrion.
Tissue Specificity	Ubiquitous, with stronger expression in tissues with high energy requirements: heart, brain, and skeletal muscle. {ECO:0000269\|PubMed:15547249}.

PAPD1 (F-12) - sc-165188 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

PAPD1 (I-15) - sc-165189 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-Poly(A) RNA polymerase, mitochondrial antibody - ab156684 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Poly(A) RNA polymerase, mitochondrial antibody (ab156684) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 50 µgLane 4 : Jurkat whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-Poly(A) RNA polymerase, mitochondrial antibody - ab154555 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Poly(A) RNA polymerase, mitochondrial antibody (ab154555) at 1/5000 dilution + HCT116 whole cell lysate at 30 µg

Anti-Poly(A) RNA polymerase, mitochondrial antibody - ab137643 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Poly(A) RNA polymerase, mitochondrial antibody (ab137643) at 1/3000 dilution + HCT116 whole cell lysate at 30 µg

Anti-Poly(A) RNA polymerase, mitochondrial antibody - ab78618 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Poly(A) RNA polymerase, mitochondrial antibody (ab78618) at 1 µg/mlLane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-MTPAP Antibody (aa532-582) - LS-C290550 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human PAPD1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PAPD1 antibody used for IP at 6 ug/mg lysate. PAPD1 was also immunoprecipitated by rabbit anti-PAPD1 antibody BL14103. For blotting immunoprecipitated PAPD1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

PAPD1 Antibody - NBP1-83856 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence: PAPD1 Antibody [NBP1-83856] - Staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli and mitochondria.

PAPD1 Antibody - NBP2-19690 from Novus Biologicals

Prices	$349.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: PAPD1 Antibody [NBP2-19690] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:5000.

PAPD1 Antibody - NBP2-22258 from Novus Biologicals

Prices	$359.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: PAPD1 Antibody [NBP2-22258] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), and Jurkat (50 ug) cells. NBP2-22258 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

PAPD1 Antibody - NBP2-19689 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: PAPD1 Antibody [NBP2-19689] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mt PAP antibody [N1C1] diluted at 1:3000.

PAPD1 Antibody - H00055149-D01P from Novus Biologicals

Prices	$399.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western Blot: PAPD1 Antibody [H00055149-D01P] - Analysis of MTPAP expression in transfected 293T cell line by MTPAP polyclonal antibody.Lane 1: PAPD1 transfected lysate(66.20 KDa).Lane 2: Non-transfected lysate.

Anti-MTPAP antibody produced in rabbit - SAB2701380 from Sigma-Aldrich

Prices	$326.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-MTPAP: <B>Cat. No. SAB2701380</B>: Western Blot analysis of MTPAP in extracts from <B>Lanes</B><BR/>A. HCT116 using <B>Cat. No. SAB2701380</B> at a 1:3000 dilution.

Anti-MTPAP antibody produced in rabbit - SAB2701372 from Sigma-Aldrich

Prices	$326.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunofluorescence</B><BR/>Anti-MTPAP: <B>Cat. No. SAB2701372</B>: Immunofluorescence analysis of methanol-fixed HeLa, using Mt PAP antibody at 1:500 dilution.

Anti-MTPAP antibody produced in rabbit - SAB1401744 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Polyclonal Anti-MTPAP, antibody produced in rabbit, purified immunoglobulin: <B>Cat. No. SAB1401744</B>: Antibody concentration: 0.1 - 5.0 μg/mL. Specific band of ~66.20 kDa using MTPAP transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-MTPAP antibody produced in rabbit - HPA038620 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-MTPAP: <B>Cat. No. HPA038620</B>: Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.

mtPAP antibody [N1C1] - GTX118983 from GeneTex

Prices	$119.00
Sizes	25 μl
Host	Rabbit
Clonality	Polyclonal

Anti-Mt PAP antibody [N1C1] used in Western blot. GTX118983

mtPAP antibody [N1C3] - GTX118738 from GeneTex

Prices	$119.00
Sizes	25 μl
Host	Rabbit
Clonality	Polyclonal

Anti-Mt PAP antibody [N1C3] used in ICC/IF. GTX118738

mtPAP antibody [1D3] - GTX70156 from GeneTex

Prices	$319.00
Sizes	100 μl
Host	Mouse
Clonality	Monoclonal
Clone	1D3

Anti-mtPAP antibody [1D3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX70156

mtPAP antibody [3D2] - GTX70136 from GeneTex

Prices	$319.00
Sizes	100 μl
Host	Mouse
Clonality	Monoclonal
Clone	3D2

Anti-mtPAP antibody [3D2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX70136

PAPD1 antibody - H00055149-D01P from Acris Antibodies

Prices	$390.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western Blot analysis of MTPAP expression in transfected 293T cell line (H00055149-T02) by MTPAP MaxPab polyclonal antibody. Lane 1: PAPD1 transfected lysate(66.20 KDa). Lane 2: Non-transfected lysate.

PAPD1 antibody - PAB14094 from Acris Antibodies

Prices	$420.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Whole cell extracts of U-2 OS cells ( 80 µg ) were analysed by Western blot using MTPAP polyclonal antibody ( Cat # PAB14094 ) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.

mtPAP - MBS270091 from MyBioSource

Prices	$360.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1D3

Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 7.5% SDS PAGE diluted at 1:1000

mtPAP - MBS270115 from MyBioSource

Prices	$360.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3D2

Rabbit anti-Human Poly(A) RNA polymerase, mitochondrial polyclonal antibody - DPABH-00683 from Creative Diagnostics

Host	Rabbit
Clonality	Polyclonal

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