Anti-Poly-ubiquitin (linkage specific K48) antibody [1001C]

• Supplier: Abcam
• Catalog: ab190061
• Prices: $370.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: 1001C
• Applications: WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant full length protein corresponding to Human Poly-ubiquitin (linkage specific K48) aa 1-76
• Description: Rabbit Monoclonal
• Gene: UBD
• Conjugate: Unconjugated

Product images

All lanes : Anti-Poly-ubiquitin (linkage specific K48) antibody [1001C] (ab190061) at 0.5 µg/mlLane 1 : Recombinant diubiquitin M1 linkageLane 2 : Recombinant diubiquitin K6 linkageLane 3 : Recombinant diubiquitin K11 linkageLane 4 : Recombinant diubiquitin K27 linkageLane 5 : Recombinant diubiquitin K29 linkageLane 6 : Recombinant diubiquitin K33 linkageLane 7 : Recombinant diubiquitin K48 linkageLane 8 : Recombinant diubiquitin K63 linkageLysates/proteins at 0.025 µg per lane.Secondaryanti-rabbit HRP (upper image); anti-mouse HRP (lower image) at 1/2000 dilutionA single band of appropriate size was detected only in the K48-linked diubiquitin lane.

All lanes : Anti-Poly-ubiquitin (linkage specific K48) antibody [1001C] (ab190061) at 0.05 µg/mlLane 1 : HepG2 cell lysate, untreatedLane 2 : HepG2 cell lysate, treated with 10 µM MG132 (4 hours)Lysates/proteins at 20 µg per lane.Secondaryanti-rabbit HRP at 1/2000 dilutionAs expected MG132 (a proteasome inhibitor) treatment resulted in the accumulation of K48-linked polyubiquitin chains attached to cellular proteins.