Western blot analysis of phosphorylated FKHR expression in NIH/3T3 (A,B), serum-treated NIH/3T3 (C) and EGF + serum-treated HeLa (D) whole cell lysates. Blots were probed with p-FKHR (Ser 256): sc-101681 preincubated with cognate phosphorylated peptide (A) and p-FKHR (Ser 256): sc-101681 (B,C,D).
p-FKHR (Ser 256): sc-101681. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.
p-FKHR (Ser 256): sc-101681. Immunofluorescence staining of methanol-fixed EGF and serum-treated HeLa cells showing nuclear localization.
p-FKHR (Ser 256): sc-101681. Western blot analysis of FKHR phosphorylation in non-transfected: sc-110760 (A) and mouse FKHR transfected: sc-178616 (B) 293 whole cell lysates.