FOXO1

Gene Symbol FOXO1
Entrez Gene 2308
Alt Symbol FKH1, FKHR, FOXO1A
Species Human
Gene Type protein-coding
Description forkhead box O1
Other Description forkhead box protein O1|forkhead box protein O1A|forkhead, Drosophila, homolog of, in rhabdomyosarcoma
Swissprots Q6NSK6 Q5VYC7 O43523 Q12778
Accessions AAC50342 AAC50343 EAX08627 Q12778 AF032885 AAC39591 AK310511 BC021981 AAH21981 BC070065 AAH70065 BT007455 AAP36123 BX648278 U02310 AAA03629 U36922 XM_011535008 XP_011533310 XM_011535009 XP_011533311 XM_011535010 XP_011533312 XR_941536 NM_002015 NP_002006
Function Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'- TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and S
Subcellular Location Cytoplasm {ECO:0000250|UniProtKB:Q9R1E0}. Nucleus {ECO:0000250|UniProtKB:Q9R1E0}. Note=Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells. In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway. Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation. Phosphorylation by NLK results in nuclear export. Translocates to the nucleus upon oxidative stress-induced phosphorylation at Ser-212 by STK4/MST1. SGK1-mediated phosphorylation also results in nuclear translocation. Retained in the nucleus under stress stimuli including oxidative stress, nutrient deprivation or nitric oxide. Retained in the nucleus on methylation. {ECO:0000250}.
Tissue Specificity Ubiquitous. {ECO:0000269|PubMed:9479491}.
Top Pathways Insulin signaling pathway, Prostate cancer, AMPK signaling pathway, Glucagon signaling pathway, Transcriptional misregulation in cancer

FoxO1 (C29H4) Rabbit mAb (PE Conjugate) - 14262 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C29H4
Flow cytometric analysis of Jurkat cells (blue) and IGROV1 cells (green) using FoxO1 (C29H4) Rabbit mAb (PE Conjugate).

Phospho-FoxO1 (Thr24)/FoxO3a (Thr32)/FoxO4 (Thr28) (4G6) Rabbit mAb - 2599 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 4G6
Western blot analysis of extracts from Jurkat cells treated with either Calyculin A (#9902) or LY294002 (#9901), NIH3T3 and COS-7 cells using Phospho-FoxO1 (Thr24)/(FoxO3a (Thr32)/FoxO4 (Thr28) (4G6) Rabbit mAb to detect FoxO1, FoxO3a and FoxO4 when phosphorylated at the Thr24, Thr32, and Thr28 positions, respectively (left panel). Total FoxO1, FoxO3a and FoxO4 were detected using FoxO1 (C29H4) Rabbit mAb (#2880), FoxO3a (75D8) Rabbit mAb (#2497) and FoxO4 Antibody (#9472), respectively (right panel).

Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody - 9464 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HT29 cells, serum starved or serum treated, using Phospho-Fox01 (Thr24)/Fox03a (Thr32) Antibody.

Phospho-FoxO1 (Ser319) Antibody - 2486 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from untreated or serum-treated COS-7 cells exogenously expressing GFP-FoxO1, using Phospho-FoxO1 (Ser319) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after Western transfer.

Phospho-FoxO1 (Ser256) Antibody - 9461 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS cells, serum starved or serum treated, using Phospho-Fox01 (Ser256) Antibody.

FoxO1 (C29H4) Rabbit mAb - 2880 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C29H4
Western blot analysis of extracts from IGROV-1 and COS-7 cells using FoxO1 (C29H4) Rabbit mAb.

FoxO1 (L27) Antibody - 9454 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from IGROV-1 cells and untreated, λ phosphatase- or FBS-treated (20 min.) HeLa cells using FoxO1 (L27) Antibody.

Phospho-FoxO1 (Ser319)/FoxO4 (Ser262) Antibody - 2487 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from serum-treated COS-7 cells exogenously expressing FoxO4 or Fox03a, using Phospho-FoxO1 (Ser319)/FoxO4 (Ser262) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after Western transfer.

FoxO1 (76E10) Rabbit mAb - 2488 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone 76E10
Western blot analysis of extracts from COS-7 cells transfected with FoxO4, GFP-FoxO1, FoxO3a or extracts from untransfected COS-7 cells (mock), using FoxO1 (76E10) Rabbit mAb (upper) or FoxO4 (#9472), FoxO1 (#9462) and FoxO3a (#9467) (lower).

FoxO1 Antibody - 9462 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

FKHR (C-9) - sc-374427 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
FKHR CRISPR/Cas9 KO Plasmid (h): sc-400137. Western blot analysis of human FKHR expression in empty vector control (A) and FKHR CRISPR/Cas9 KO Plasmid transfected (B,C) HeLa whole cell lysates. Blot probed with FKHR (C-9): sc-374427. Actin (I-19): sc-1616 used as specificity and loading control.

FKHR (C-20) - sc-9808 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of FKHR expression in 3611-RF nuclear extract (A) and CTLL-2 whole cell lysate (B). Antibodies tested include FKHR (C-20): sc-9808 (A) and FKHR (H-128): sc-11350 (B).

FKHR (C-15) - sc-34890 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
FKHR (C-15): sc-34890. Western blot analysis of human recombinant FKHR fusion protein.

FKHR (H-128) - sc-11350 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
FKHR (H-128): sc-11350. Western blot analysis of FKHR expression in serum-starved, then serum-treated NIH/3T3 (A) and untreated 3T3-L1 (B) whole cell lysates.

FKHR (H-130) - sc-67140 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
FKHR (H-130): sc-67140. Western blot analysis of FKHR expression in non-transfected 293: sc-110760 (A), mouse FKHR transfected 293: sc-178616 (B) and TE 671 (C) whole cell lysates.

FKHR (N-13) - sc-34891 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
FKHR (N-13): sc-34891. Western blot analysis of human recombinant FKHR fusion protein.

FKHR (N-18) - sc-9809 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
FKHR (N-18): sc-9809. Western blot analysis of FKHR expression in non-transfected: sc-110760 (A) and mouse FKHR transfected: sc-178616 (B) 293 whole cell lysates.

FKHR (Y-10) - sc-12892 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

FKHR (S-15) - sc-34893 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
FKHR (S-15): sc-34893. Western blot analysis of FKHR expression in rat liver tissue extract.

Ac-FKHR (D-19) - sc-49437 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p-FKHR (Thr 24) - sc-22161 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-FKHR (Thr 24): sc-22161. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of Purkinje cells.

p-FKHR (Ser 329) - sc-19808 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p-FKHR (Ser 319) - sc-19807 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p-FKHR (Ser 256) - sc-16307 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p-FKHR (Ser 256) - sc-101681 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of phosphorylated FKHR expression in NIH/3T3 (A,B), serum-treated NIH/3T3 (C) and EGF + serum-treated HeLa (D) whole cell lysates. Blots were probed with p-FKHR (Ser 256): sc-101681 preincubated with cognate phosphorylated peptide (A) and p-FKHR (Ser 256): sc-101681 (B,C,D).
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