Anti-PON2 antibody [AF3E6]

• Supplier: Abcam
• Catalog: ab85340
• Prices: $384.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone: AF3E6
• Applications: FC WB IP
• Species Reactivities: Human
• Antigen: His tagged recombinant Human PON2 protein purified from E
• Description: Mouse Monoclonal
• Gene: PON2
• Conjugate: Unconjugated

Product images

Overlay histogram showing HepG2 cells stained with ab85340 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85340, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG ( 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-PON2 antibody [AF3E6] (ab85340) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : 293T cell lysate

All lanes : Anti-PON2 antibody [AF3E6] (ab85340) at 1/1000 dilutionLane 1 : PON1 Recombinant ProteinLane 2 : PON2 Recombinant ProteinLane 3 : PON3 Recombinant ProteinLane 4 : HeLa Cell LysateLane 5 : HepG2 Cell LysateLane 6 : U87mg Cell Lysate

All lanes : Anti-PON2 antibody [AF3E6] (ab85340) at 1/1000 dilutionLane 1 : HeLa Cell LysateLane 2 : PC12 Cell LysateLane 3 : H9C2 Cell LysateLane 4 : C6 Cell LysateLane 5 : L929 Cell LysateLane 6 : NIH3T3 Cell LysateLane 7 : Rat Liver LysateLane 8 : Mouse Liver LysateSecondaryMouse-specific secondary at 1/5000 dilution