Anti-PORCN antibody (ab105543) at 1 µg/ml + HepG2 cell lysate at 10 µg
ab105543 staining PORCN in murine uterus tissue by Immunohistochemistry (PFA perfusion fixed frozen sections).Tissue was fixed in 4% PFA, blocked with 2% goat serum and then incubated with ab105543 at a 1/300 dilution at 4°C overnight. The secondary used was used at a 1/1000 dilution. Counterstain P.I. – 1/5000.
ICC/IF image of ab105543 stained Panc-1 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105543, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.