All lanes : Anti-PPAR gamma [EP4394(N)] antibody (ab191407) at 1/1000 dilutionLane 1 : 3T3-L1 cell lysateLane 2 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-PPAR gamma [EP4394(N)] antibody (ab191407) at 1/1000 dilutionLane 1 : Human adipose tissue lysateLane 2 : Human fetal liver tissue lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-PPAR gamma [EP4394(N)] antibody (ab191407) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : Raw264.7 cell lysateLane 3 : PC12 cell lysateLane 4 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution