Anti-PPAR gamma antibody

• Supplier: Abcam
• Catalog: ab19481
• Prices: $390.00
• Sizes: 200 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P WB
• Species Reactivities: Mouse, Rat, Chicken, Hamster, Bovine, Dog, Human, Pig
• Antigen: Synthetic peptide (Human) (C terminal) from a region between AA 420 and 460 of the PPARgamma protein
• Description: Rabbit Polyclonal
• Gene: PPARG
• Conjugate: Unconjugated

Product images

ab19481 staining PPARγ in U-87 MG cells treated with ciglitazone (ab141139), by ICC/IF. Increase of PPARγ cytoplasmic expression correlates with increased concentration of ciglitazone, as described in literature.The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab141139 (ciglitazone) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab19481 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

ab19481 staining CHO K1 cells expressing Human PPAR gamma by ICC/IF.  Cells were formaldehyde fixed and permeabilized in 0.1 % Triton X-100 prior to blocking with TBS + 3% Dried Milk + 0.1 % Triton X-100 for 20 minutes at 22°C.  The primary anitbody was diluted 1/50 and incubated with the sample for 1 hour at 22°C.  A FITC conjugated goat anti-rabbit antibody diluted 1/400 was used as the secondary.  The cells in the top image clearly show punctate cytoplasmic and nuclear localization of Human PPAR gamma when grown in the presence of F12 + 1.5% Charcoal/Dextran FBS. See Abreview

Ab19481 staining Human normal placenta. Staining is localized to nuclear compartment.Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

ICC/IF image of ab19481 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19481, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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