Anti-PPAR gamma antibody

• Supplier: Abcam
• Catalog: ab59256
• Prices: $379.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF ELISA IHC-P
• Species Reactivities: Mouse, Human
• Antigen: Synthetic non-phosphopeptide derived from human PPAR gamma around the phosphorylation site of serine 112 (P-A-SP-P-P)
• Description: Rabbit Polyclonal
• Gene: PPARG
• Conjugate: Unconjugated

Product images

ab59256, at 1/50 dilution, staining PPAR gamma in paraffin embedded human placenta tissue by Immunohistochemistry in the absence or presence of the immunising peptide.

ICC/IF image of ab59256 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59256, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Developmental programming: effect of prenatal steroid excess on intraovarian - Developmental programming: effect of prenatal steroid excess on intraovarian

Ortega HH, Rey F, Velazquez MM, Padmanabhan V. Biol Reprod. 2010 Jun;82(6):1065-75.

The effect of the PPAR-gamma agonist rosiglitazone on neuroblastoma SK-N-SH cells - The effect of the PPAR-gamma agonist rosiglitazone on neuroblastoma SK-N-SH cells

Krieger-Hinck N, Schumacher U, Muller A, Valentiner U. Oncol Res. 2010;18(8):387-93.