Anti-PPID antibody - N-terminal (ab135717) at 1/50 dilution + NIH-3T3 cell line lysates at 35 µg
All lanes : Anti-PPID antibody - N-terminal (ab135717) at 1/50 dilutionLane 1 : 293 cell lysates non transfectedLane 2 : 293 cell lysates transiently transfected with the PPID gene. Lysates/proteins at 2 µg per lane.
Immunohistochemical analysis of PPID expression in paraffin embedded, formalin fixed Human brain tissue labelled with ab135717 at 1/50 dilution. This was followed by incubation with a peroxidase conjugated secondary antibody and DAB staining.
Flow cytometric analysis of PPID expression in K562 cells labelled with ab135717 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.