All lanes : Anti-PPM1A antibody (ab154489) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLysates/proteins at 30 µg per lane.
Anti-PPM1A antibody (ab154489) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling PPM1A with ab154489 at 1/500 dilution.
Immunofluorescent analysis of methanol-fixed (iced cold; 5mins) A431 cells labeling PPM1A with ab154489 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.