ELISA analysis using ab135249 with different concentrations of antigen.
Anti-PPM1A antibody [7F12] (ab135249) at 1/500 dilution + Human PPM1A recombinant protein.
All lanes : Anti-PPM1A antibody [7F12] (ab135249) at 1/500 dilutionLane 1 : HEK293 cell lysateLane 2 : PPM1A (amino acids 202-382)-hIgGFc transfected HEK293 cell lysate.
All lanes : Anti-PPM1A antibody [7F12] (ab135249) at 1/500 dilutionLane 1 : Jurkat cell lysateLane 2 : Jurkat cell lysateLane 3 : A431 cell lysateLane 4 : HeLa cell lysateLane 5 : HEK293 cell lysateLane 6 : Raji cell lysateLane 7 : MCF-7 cell lysateLane 8 : COS7 cell lysate
Flow cytometric analysis of HeLa cells using ab135249 at 1/200 dilution (green) or a negative control (red).