Anti-PPM1A antibody [p6c7]

• Supplier: Abcam
• Catalog: ab14824
• Prices: $400.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone: p6c7
• Applications: IHC-P ELISA WB
• Species Reactivities: Mouse, Human
• Antigen: Recombinant full length protein (Human)
• Description: Mouse Monoclonal
• Gene: PPM1A
• Conjugate: Unconjugated

Product images

Anti-PPM1A antibody [p6c7] (ab14824) at 1/1000 dilution + HeLa whole cell lysateSecondaryHRP conjugated anti-mouse antibodydeveloped using the ECL techniquePerformed under reducing conditions.

Ab14824 staining human colon. Staining is localised to cytoplasm.Left panel: with primary antibody at 4ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Product References

Role of protein phosphatase magnesium-dependent 1A and anti-protein phosphatase - Role of protein phosphatase magnesium-dependent 1A and anti-protein phosphatase

Kim YG, Sohn DH, Zhao X, Sokolove J, Lindstrom TM, Yoo B, Lee CK, Reveille JD, Taurog JD, Robinson WH. Arthritis Rheumatol. 2014 Oct;66(10):2793-803.

Phosphatase PPM1A negatively regulates P-TEFb function in resting CD4(+) T cells - Phosphatase PPM1A negatively regulates P-TEFb function in resting CD4(+) T cells

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Hypoxia-activated Smad3-specific dephosphorylation by PP2A. - Hypoxia-activated Smad3-specific dephosphorylation by PP2A.

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Dihydrosphingosine 1-phosphate has a potent antifibrotic effect in scleroderma - Dihydrosphingosine 1-phosphate has a potent antifibrotic effect in scleroderma

Bu S, Asano Y, Bujor A, Highland K, Hant F, Trojanowska M. Arthritis Rheum. 2010 Jul;62(7):2117-26.

PPM1A and PPM1B act as IKKbeta phosphatases to terminate TNFalpha-induced - PPM1A and PPM1B act as IKKbeta phosphatases to terminate TNFalpha-induced

Sun W, Yu Y, Dotti G, Shen T, Tan X, Savoldo B, Pass AK, Chu M, Zhang D, Lu X, Fu S, Lin X, Yang J. Cell Signal. 2009 Jan;21(1):95-102.

TGF-beta signalling is regulated by Schnurri-2-dependent nuclear translocation of - TGF-beta signalling is regulated by Schnurri-2-dependent nuclear translocation of

Shukla A, Malik M, Cataisson C, Ho Y, Friesen T, Suh KS, Yuspa SH. Nat Cell Biol. 2009 Jun;11(6):777-84.

Opposite effects of dihydrosphingosine 1-phosphate and sphingosine 1-phosphate on - Opposite effects of dihydrosphingosine 1-phosphate and sphingosine 1-phosphate on

Bu S, Kapanadze B, Hsu T, Trojanowska M. J Biol Chem. 2008 Jul 11;283(28):19593-602.

PPM1A functions as a Smad phosphatase to terminate TGFbeta signaling. - PPM1A functions as a Smad phosphatase to terminate TGFbeta signaling.

Lin X, Duan X, Liang YY, Su Y, Wrighton KH, Long J, Hu M, Davis CM, Wang J, Brunicardi FC, Shi Y, Chen YG, Meng A, Feng XH. Cell. 2006 Jun 2;125(5):915-28.

Protein serine/threonine phosphatase PPM1A dephosphorylates Smad1 in the bone - Protein serine/threonine phosphatase PPM1A dephosphorylates Smad1 in the bone

Duan X, Liang YY, Feng XH, Lin X. J Biol Chem. 2006 Dec 1;281(48):36526-32. Epub 2006 Aug 24.