Anti-PPM1D antibody
| Name | Anti-PPM1D antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab31270 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat, Xenopus, Zebrafish |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human PPM1D |
| Description | Rabbit Polyclonal |
| Gene | PPM1D |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-PPM1D antibody (ab31270) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Immunohistochemical staining using ab31720 in paraffin-embedded HCC and non-cancerous liver tissues, 5 µm sections, at a dilution of 1/1000. (a) Negative ppm1d staining in normal liver tissue. (b) Negative staining of HCC tissue without ab31720. (c) Ppm1d-positive non-cancerous liver tissue. (d) High expression of ppm1d in HCC tissue. Antigen retrieval was performed in a microwave with 0.1 M sodium citrate buffer (pH 6.0). The secondary antibody was a HRP conjugated compact polymer system and the samples were counter stained with DAB. These images were taken on a Keyence BZ-8000 digital microscope at a magnification of x200 and the scale bar represents 100 µm.
IHC image of ab31270 staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31270, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ICC/IF image of ab31270 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31270, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Protein phosphatase magnesium-dependent 1delta (PPM1D) mRNA expression is a - Protein phosphatase magnesium-dependent 1delta (PPM1D) mRNA expression is a
Li GB, Zhang XL, Yuan L, Jiao QQ, Liu DJ, Liu J. PLoS One. 2013;8(3):e60775.
Nutlin-3a, an MDM2 antagonist and p53 activator, helps to preserve the - Nutlin-3a, an MDM2 antagonist and p53 activator, helps to preserve the
Zajkowicz A, Krzesniak M, Matuszczyk I, Glowala-Kosinska M, Butkiewicz D, Rusin M. Mol Biol Rep. 2013 Aug;40(8):5013-26.