Detection of PPP2CB by Western Blot of Immunprecipitate.293T cell lysate immunoprecipitated using ab168371 at 1/10 dilution.For WB detection ab168371 at 1/10000 dilution was used.HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow Cytometric analysis of permeabilized NIH 3T3 cells labeling PPP2CB with ab168371 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
Immunofluorescent analysis of 293T cells labeling PPP2CB with ab168371 at 1/50 dilution.
All lanes : Anti-PPP2CB antibody [EPR11786(B)] (ab168371) at 1/10000 dilutionLane 1 : 293T lysateLane 2 : HeLa lysateLane 3 : Human fetal brain lysateLane 4 : Human fetal heart lysateLane 5 : NIH3T3 lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution