All lanes : Anti-PPP2CB antibody [EPR11787(B)] (ab168350) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : A431 cell lysateLane 4 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/2000 dilution
Immunofluorescent analysis of A431 cells labeling PPP2CB with ab168350 at 1/50 dilution.
Anti-PPP2CB antibody [EPR11787(B)] (ab168350) at 1/10000 dilution + immunoprecipitation pellet from HeLa cell lysateSecondaryHRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG