To detect Rat RANTES by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat RANTES (XP-5258Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat RANTES.
To detect Rat RANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat RANTES is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat RANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat RANTES is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained colchicine injected rat brain (somatosensory cortex) tissue. The primary antibody was incubated at 1.0 mg/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.