Anti-PRAME antibody (ab89097) at 1 µg/ml + HeLa cell lysate at 50 µg
All lanes : Anti-PRAME antibody (ab89097) at 1 µg/mlLane 1 : PRAME transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.
ab89097 staining PRAME in Human Melanoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a Tris/EDTA buffer. Samples were incubated with primary antibody (1/40) for 1 hour 30 minutes at 28°C. See Abreview