Immunohistochemical analysis of formalin fixed, paraffin embedded Human testis tissue labelling PRAME with ab135600 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells labelling PRAME with ab135600 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC conjugated Goat anti-rabbit secondary antibodies were used for the analysis.
Anti-PRAME antibody - C-terminal (ab135600) at 1/50 dilution + K562 cell lysate at 35 µg