All lanes : Anti-PRAS40 antibody [EPR6263(2)] (ab151719) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : SHSY5Y cell lysateLane 3 : HeLa cell lysateLane 4 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PRAS40 with ab151719 at 1/50 dilution.
Flow cytometric analysis of permeabilized 293T cells labeling PRAS40 with ab151719 at 1/10 dilution (red), compared to a nonspecific control antibody (green).