AKT1S1

Gene Symbol	AKT1S1
Entrez Gene	84335
Alt Symbol	Lobe, PRAS40
Species	Human
Gene Type	protein-coding
Description	AKT1 substrate 1 (proline-rich)
Other Description	40 kDa proline-rich AKT substrate|proline-rich AKT1 substrate 1
Swissprots	A8MTQ1 Q96B36 Q9BWR5 Q96BI4 J3KPM3 B2RE93 Q96NG2 Q96IK7
Accessions	EAW52568 EAW52569 EAW52570 EAW52571 Q96B36 AK055511 BAB70937 AK092610 BAG52583 AK123687 AK226004 AK316603 BAG38190 BC000031 AAH00031 BC007416 AAH07416 BC015562 AAH15562 BC016043 AAH16043 BC022241 BC051844 AAH51844 DA005259 DA476468 DA542314 DA848626 NM_001098632 NP_001092102 NM_001098633 NP_001092103 NM_001278159 NP_001265088 NM_001278160 NP_001265089 NM_032375 NP_115751
Function	Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phospho
Subcellular Location	Cytoplasm, cytosol {ECO:0000250}. Note=Found in the cytosolic fraction of the brain. {ECO:0000250}.
Tissue Specificity	Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293). {ECO:0000269|PubMed:12524439, ECO:0000269|PubMed:16174443}.
Top Pathways	AMPK signaling pathway, mTOR signaling pathway

• Antibodies
• Assays/Kits
• Proteins/Peptides
• siRNAs/Functional Genomics
• cDNA Clones
• Cell/Tissue/Blood Products

PRAS40 (D23C7) XP ® Rabbit mAb (Biotinylated) - 8858 from Cell Signaling Technology Prices $329.00 Sizes 100 µl (10 western blots) Host Rabbit Clonality Monoclonal Clone D23C7

Phospho-PRAS40 (Thr246) (D4D2) XP ® Rabbit mAb - 13175 from Cell Signaling Technology Prices $119.00, $324.00 Sizes 20 µl (2 western blots), 100 µl (10 western blots) Host Rabbit Clonality Monoclonal Clone D4D2

Phospho-PRAS40 (Thr246) Antibody - 2640 from Cell Signaling Technology Prices $287.00 Sizes 100 µl (10 western blots) Host Rabbit Clonality Polyclonal

Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb - 2997 from Cell Signaling Technology Prices $109.00, $292.00 Sizes 20 µl (2 western blots), 100 µl (10 western blots) Host Rabbit Clonality Monoclonal Clone C77D7

Phospho-PRAS40 (Ser183) Antibody - 5936 from Cell Signaling Technology Prices $287.00 Sizes 100 µl (10 western blots) Host Rabbit Clonality Polyclonal

PRAS40 (D23C7) XP ® Rabbit mAb - 2691 from Cell Signaling Technology Prices $109.00, $277.00 Sizes 20 µl (2 western blots), 100 µl (10 western blots) Host Rabbit Clonality Monoclonal Clone D23C7

PRAS40 Antibody - 2610 from Cell Signaling Technology Prices $246.00 Sizes 100 µl (10 western blots) Host Rabbit Clonality Polyclonal

PRAS40 (H-216) - sc-67042 from Santa Cruz Biotechnology Prices $279.00 Sizes 200 µg/ml Host Rabbit Clonality Polyclonal

PRAS40 (C-20) - sc-32628 from Santa Cruz Biotechnology Prices $279.00 Sizes 200 µg/ml Host Goat Clonality Polyclonal

PRAS40 (S-20) - sc-32627 from Santa Cruz Biotechnology Prices $279.00 Sizes 200 µg/ml Host Goat Clonality Polyclonal

p-PRAS40 (Thr 246) - sc-32629 from Santa Cruz Biotechnology Prices $279.00 Sizes 200 µg/ml Host Rabbit Clonality Polyclonal

Anti-PRAS40 (phospho T246) antibody - ab59171 from Abcam Prices $370.00 Sizes 100 µg Host Rabbit Clonality Polyclonal

Anti-PRAS40 (phospho T246) antibody [EPR6262-107] - ab134084 from Abcam Prices $313.00 Sizes 100 µl Host Rabbit Clonality Monoclonal Clone EPR6262-107

Anti-PRAS40 antibody - ab155174 from Abcam Prices $370.00 Sizes 100 µl Host Rabbit Clonality Polyclonal

Anti-PRAS40 antibody - ab59438 from Abcam Prices $378.00 Sizes 100 µg Host Rabbit Clonality Polyclonal

Anti-PRAS40 antibody - ab72321 from Abcam Prices $370.00 Sizes 100 µl Host Rabbit Clonality Polyclonal

Anti-PRAS40 antibody - ab80855 from Abcam Prices $370.00 Sizes 100 µl Host Rabbit Clonality Polyclonal

Anti-PRAS40 antibody - ab123294 from Abcam Prices $370.00 Sizes 400 µl Host Rabbit Clonality Polyclonal

Anti-PRAS40 antibody - ab140387 from Abcam Prices $370.00 Sizes 100 µl Host Rabbit Clonality Polyclonal

Anti-PRAS40 antibody [73P21] - ab52561 from Abcam Prices $370.00 Sizes 50 µg Host Mouse Clonality Monoclonal Clone 73P21

Anti-PRAS40 antibody [EPR6262] - ab134158 from Abcam Prices $313.00 Sizes 100 µl Host Rabbit Clonality Monoclonal Clone EPR6262

Anti-PRAS40 antibody [EPR6263(2)] - ab151719 from Abcam Prices $357.00 Sizes 100 µl Host Rabbit Clonality Monoclonal Clone EPR6263(2)

Anti-PRAS40 antibody [EPR6402] - ab133584 from Abcam Prices $313.00 Sizes 100 µl Host Rabbit Clonality Monoclonal Clone EPR6402

Anti-PRAS40 antibody - C-terminal - ab181408 from Abcam Prices $370.00 Sizes 100 µg Host Rabbit Clonality Polyclonal

Anti-AKT1S1 / PRAS40 Antibody (phospho-Thr246, clone EPR6262) - LS-C138982 from LifeSpan Bioscience Prices $395.00 Sizes 100 µl Host Rabbit Clonality Monoclonal Clone EPR6262

Search more