![Overlay histogram showing Ramos cells stained with ab96479 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab96479, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/15593_PRDM1Blimp1-Primary-antibodies-ab96479-2.jpg)
Overlay histogram showing Ramos cells stained with ab96479 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab96479, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-PRDM1/Blimp1 antibody [5E7] (ab96479) at 1/500 dilutionLane 1 : Raji cell lysateLane 2 : Raji cell lysateLane 3 : L1210 cell lysateLane 4 : TPH1 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/15592_PRDM1Blimp1-Primary-antibodies-ab96479-1.jpg)
All lanes : Anti-PRDM1/Blimp1 antibody [5E7] (ab96479) at 1/500 dilutionLane 1 : Raji cell lysateLane 2 : Raji cell lysateLane 3 : L1210 cell lysateLane 4 : TPH1 cell lysate