Anti-Presenilin 2 antibody [APS 21]
| Name | Anti-Presenilin 2 antibody [APS 21] |
|---|---|
| Supplier | Abcam |
| Catalog | ab15548 |
| Prices | $385.00 |
| Sizes | 200 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | APS 21 |
| Applications | WB ICC/IF ICC/IF IHC-P ELISA |
| Species Reactivities | Mouse, Rat, Human, Primate |
| Antigen | Synthetic peptide corresponding to Human Presenilin 2 aa 31-45 |
| Description | Mouse Monoclonal |
| Gene | PSEN2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunocytochemistry/Immunofluorescence analysis of NCI-H460 cells labeling Presenilin 2 (green) with ab15548 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Presenilin 2 (green) with ab15548 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
Immunocytochemistry/Immunofluorescence analysis of A2058 cells labeling Presenilin 2 (green) with ab15548 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
![All lanes : Anti-Presenilin 2 antibody [APS 21] (ab15548)Lane 1 : Cell lysate prepared from control siRNA treated Mouse C2C12 myoblasts. Lane 2 : Cell lysates prepared from PS2 siRNA treated Mouse C2C12 myoblasts.SecondaryHRP-conjugated secondary antibody was used for visualisation by chemiluminescence.Image from Ono Y et al, J Cell Sci. 2009 Dec 15;122(Pt 24):4427-38. Epub 2009 Nov 17, Fig 7.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/15780_Presenilin-2-Primary-antibodies-ab15548-1.jpg)
All lanes : Anti-Presenilin 2 antibody [APS 21] (ab15548)Lane 1 : Cell lysate prepared from control siRNA treated Mouse C2C12 myoblasts. Lane 2 : Cell lysates prepared from PS2 siRNA treated Mouse C2C12 myoblasts.SecondaryHRP-conjugated secondary antibody was used for visualisation by chemiluminescence.Image from Ono Y et al, J Cell Sci. 2009 Dec 15;122(Pt 24):4427-38. Epub 2009 Nov 17, Fig 7.
Immunohistochemistry was performed on normal biopsies of deparaffinized Human kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Presenilin 2 ab15548 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Product References
Thapsigargin affects presenilin-2 but not presenilin-1 regulation in SK-N-BE - Thapsigargin affects presenilin-2 but not presenilin-1 regulation in SK-N-BE
Rivabene R, Visentin S, Piscopo P, De Nuccio C, Crestini A, Svetoni F, Rosa P, Confaloni A. Exp Biol Med (Maywood). 2014 Feb;239(2):213-24.
Presenilin-1 acts via Id1 to regulate the function of muscle satellite cells in a - Presenilin-1 acts via Id1 to regulate the function of muscle satellite cells in a
Ono Y, Gnocchi VF, Zammit PS, Nagatomi R. J Cell Sci. 2009 Dec 15;122(Pt 24):4427-38.