Anti-Presenilin 2 antibody [EP1515Y]
| Name | Anti-Presenilin 2 antibody [EP1515Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab51249 |
| Prices | $99.00, $395.00 |
| Sizes | 10 µl, 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1515Y |
| Applications | WB IHC-P ICC/IF ICC/IF IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues on the C terminus of human Presenilin 2 |
| Description | Rabbit Monoclonal |
| Gene | PSEN2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized JAR (Human placenta choriocarcinoma cell line) cells labeling Presenilin with ab51249 at 1/250 dilution, followed by Anti-rabbit Alexa Fluor® 488 (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasm staining on JAR cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab51249 at 1/250 dilution followed by Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution.
Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized A-673 (Human muscle Ewing's Sarcoma cell line) cells labeling Presenilin with ab51249 at 1/250 dilution, followed by Anti-rabbit Alexa Fluor® 488 (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining on A-673 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab51249 at 1/250 dilution followed by Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution.
Ab51249 (1:50) staining human Presenilin 2 in human kidney tissue by immunohistochemistry using paraffin embedded tissue.Ab51249 (1:50) staining human Presenilin 2 in human kidney tissue by immunohistochemistry using paraffin embedded tissue.
![Anti-Presenilin 2 antibody [EP1515Y] (ab51249) at 1/200000 dilution + PC-12 cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/15789_ab51249_1.jpg)
Anti-Presenilin 2 antibody [EP1515Y] (ab51249) at 1/200000 dilution + PC-12 cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution
![Anti-Presenilin 2 antibody [EP1515Y] (ab51249) at 1/1000 dilution (for 16 hours at 4°C) + Human HeLa cells - whole cell lysate at 30 µgSecondaryAn HRP-conjugated Goat anti-rabbit IgG at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/15790_Presenilin-2-Primary-antibodies-ab51249-4.jpg)
Anti-Presenilin 2 antibody [EP1515Y] (ab51249) at 1/1000 dilution (for 16 hours at 4°C) + Human HeLa cells - whole cell lysate at 30 µgSecondaryAn HRP-conjugated Goat anti-rabbit IgG at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Presenilin was immunoprecipitated from 1mg of HeLa whole cell extract with ab51249 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab51249 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Input: HeLa whole cell extract 10 µg. IP (+): ab51249 IP in HeLa whole cell extract. IP (-): Rabbit monoclonal IgG (ab172730) instead of ab51249 in HeLa whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Presenilin was immunoprecipitated from 1mg of PC-12 (rat adrenal gland pheochromocytoma) whole cell extract with ab51249 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab51249 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Input: PC-12 whole cell extract 10 µg.IP (+): ab51249 IP in PC-12 whole cell extract.IP (-): Rabbit monoclonal IgG (ab172730) instead of ab51249 in PC-12 whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Product References
An ancestral non-proteolytic role for presenilin proteins in multicellular - An ancestral non-proteolytic role for presenilin proteins in multicellular
Ludtmann MH, Otto GP, Schilde C, Chen ZH, Allan CY, Brace S, Beesley PW, Kimmel AR, Fisher P, Killick R, Williams RS. J Cell Sci. 2014 Apr 1;127(Pt 7):1576-84.
The endocannabinoid, anandamide, augments Notch-1 signaling in cultured cortical - The endocannabinoid, anandamide, augments Notch-1 signaling in cultured cortical
Tanveer R, Gowran A, Noonan J, Keating SE, Bowie AG, Campbell VA. J Biol Chem. 2012 Oct 5;287(41):34709-21.