Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab3763 to PRMT2 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 10 µl of ab3763 and 2x106 cells were used in each ChIP experiment. Comments: ab3763 appears to work in ChIP. It is difficult to be sure about the relevance of the results since we do not know what we could expect. If you compare ab3667 and 3763 to PRMT2, the results are actually not too bad even if they give slightly different profiles at 30 and 60 min.
ab3763 staining PRMT2 in human breast carcinoma (BC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).